Democratization and accessibility of complex engineering tools
Our mission is to develop advanced engineering tools that are both affordable and accessible to non-engineering laboratories. In collaboration with experts in developmental biology, the microbiome, infectious diseases, and clinical research, we have created a suite of innovative technologies designed to enhance the study of human physiology and disease. Use the links below to request more information or explore potential collaborations.
Zonal patterning of extracellular matrix and stromal cell populations along a perfusable cellular microchannel, B Chernokal*, BJ Ferrick*, JP Gleghorn, Lab on a Chip, 2024, 24, 5238 - 5250. *indicates equal contribution
We have developed and validated a simple strategy to create an in vitro microphysiological system with regionally patterned stromal cell populations and hydrogel properties along the length of a perfused continuous tubular epithelium. This highly customizable system has wide utility for modeling epithelial and endothelial tissue interactions with heterogeneous hydrogel compositions and/or stromal cell populations to better dissect the specific microenvironmental factors that govern developmental tissue patterning.
Click here for more information or to inquire about a collaboration
Zonal Patterning Device
In-house required equipment:
Incubator
Biosafety cabinet
Peristaltic pump with flow rate control down to at least 0.01 mL/min
Modular Microphysiological Device
Democratizing Organ-on-Chip Technologies with a Modular, Reusable, and Perfusion-Ready Microphysiological System, DJ Minahan*, KM Nelson*, F Ribeiro, BJ Ferrick, A Zurzolo, K Byers, and JP Gleghorn, BioRxiv, doi: 10.1101/2025.04.30.651503, *indicates equal contribution
We have developed and validated a simple strategy to create a two layer in vitro microphysiological system within a one-time-use cell culture insert and a reusable cassette to enable fluid flow. The system enables easier cell culture than PDMS based devices and can be irreversibly sealed to enable access to the cells for assays. This highly customizable system has wide utility for modeling transport across a cell barrier and cell-cell interactions to better study physiological and pathogenic processes.
Click here for more information or to inquire about a collaboration
In-house required equipment:
Incubator (hypoxic preferred)
Biosafety cabinet
Multichannel peristaltic pump with flow rate control down to at least 0.01 mL/min
